Journal Papers Methylation status of p16 INK4A tumor suppressor gene in Iranian patients with sporadic breast cancer


Introduction: p16 INK4A is a tumor suppressor encoding the Cdk inhibitor protein, which acts to repress Cdk4/6 and pRb phosphorylation. p16 INK4A gene can be inactivated by a variety of events, including promoter hypermethylation. Materials and methods: To investigate the methylation status of the p16 INK4A gene in Iranian patients with breast carcinoma, promoter methylation was studied by methylation-specific PCR (MSP) and restriction enzyme-related PCR (REP). In addition, p16 INK4A promoter was analyzed by PCR-SSCP in order to detection of mutation and single nucleotide polymorphisms. Results: Analysis of 70 patients by MPS and REP showed hypermethylation of p16 INK4A promoter in 35.7% (25/70) and 40% (28/70) of samples, respectively. Comparison of the molecular data and pathological information of the samples suggested that p16 INK4A gene might be inactivated at the early stages in breast cancer. Conclusion: Therefore, it could be suggested that hypermethylation of p16 INK4A promoter is one of the epigenetic factors affecting the progress of sporadic breast carcinogenesis in Iranian patients.

Paper Details


S. Vallian,  M. Sedaghat,  I. Nassiri,  A. Frazmand


Journal of Cancer Research and Clinical Oncology , 135, , 991-996