PhD Thesis In silico modeling of cell polarity establishment and cell division initiation regulation networks of fission yeast cells


Rod shaped wild fission yeast cells grow only at one tip in G1 phase; later in G2 they switch over to bipolar growth which is controlled by conserved signaling pathways. The molecules that regulate cell division and differentiation are conserved among eukaryotes and fission yeast is an ideal organism to investigate these signaling pathways. In fission yeast the localization of growth zone is governed by microtubules and induction of growth is regulated by actin machinery. Actin is used for polarized growth during interphase and it is relocalized in the middle of the cell in mitosis to induce cell division. Several studies have been investigating how cell cycle and changes in actin localization coordinate with each other and which are the key proteins this coupling, still we lack a complete picture. In order to understand this connection between the cell cycle and cell growth, we performed network analysis on the predicted protein-protein interaction network of fission yeast and categorized the key core components and the proteins that connect these cellular processes. Furthermore, we constructed mathematical models of different levels of complexity to investigate the symmetrical localization of some of the key regulatory proteins of the timing of cell division.

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A. Bajpai